| Course Unit Title | Course Unit Code | Type of Course Unit | Level of Course Unit | Year of Study | Semester | ECTS Credits |
|---|---|---|---|---|---|---|
| Enzyme Kinetics | KIM508 | Elective | Master's degree | 1 | Spring | 8 |
Name of Lecturer(s)
Prof. Dr. Yonca DUMAN
Prof. Dr. Neslihan ŞAKİ
Associate Prof. Dr. Mustafa Oğuzhan KAYA
Learning Outcomes of the Course Unit
2) Recognize of the Integrated form of the Michaelis-Menten kinetics
2) Distinguish between of rapid equilibrium partial and mixed type inhibition and linear mixed-type inhibitions
3) Explain of Enzyme Activation, Kinetics of bisubstrate enzymatic reactions, Effect of pH and temperature on enzyme kinetics, Irreversible inactivation kinetics of enzyme.
4) Explain of factors responsible for the catalytic efficiency and kinetics of unireactant enzymes
5) Identify of Simple inhibition systems and Substrate inhibition.
Program Competencies-Learning Outcomes Relation
| Program Competencies | |||||||
| 1 | 2 | 3 | 4 | 5 | 6 | ||
| Learning Outcomes | |||||||
| 2 | Middle | No relation | No relation | No relation | No relation | No relation | |
| 2 | Middle | No relation | No relation | No relation | No relation | No relation | |
| 3 | Middle | No relation | No relation | No relation | No relation | No relation | |
| 4 | Middle | No relation | No relation | No relation | No relation | No relation | |
| 5 | Middle | No relation | No relation | No relation | No relation | No relation | |
Mode of Delivery
Face to Face
Prerequisites and Co-Requisites
None
Recommended Optional Programme Components
Enzymology
Course Contents
Factors of catalytic efficiency of enzymes, Kinetic of single substrate enzymes; Rapid equilibrium and Briggs-Haldane 's steady state approach,Substrate concentration versus velocity and reaction order, determination of Km and Vmax by graphically, Integration of Michael-Menten kinetic, Simple inhibition systems: competitive, non-competitive and un-competitive inhibitions; contaminant inhibitor effect on initial reaction rate of enzymes, very tightly bound inhibitors, substrate inhibition, rapid equilibrium and mixed inhibitions, partial competitive and non-competitive inhibitiohs, Linear mixed type inhibitions and hyperblolic mixed type inhibitions, Enzyme activation, pH and temperature effect on enzyme kinetic, ırreversible enzyme kinetic.
Weekly Schedule
1) Factors responsible for the catalytic efficiency of enzymes2) Kinetics of unireactant enzymes: Rapid equilibrium and Briggs-Haldane steady-state approach,
3) Velocity versus substrate concentration curve and reaction order
4) Simple inhibition systems: Competitive, non-competitive and un-competitive inhibitions
5) Simple inhibition systems: Competitive, non-competitive and un-competitive inhibitions
6) Effect of contaminating inhibitors on the initial velocity of enzymes
7) Tightly bound inhibitors; Substrate inhibition
8) Rapid equilibrium partial and mixed type inhibition: Partial competitive inhibition
9) Rapid equilibrium partial and mixed type inhibition: Partial non-competitive inhibitions
10) Linear mixed-type inhibitions
11) Hyperbolic mixed type inhibitons
12) Enzyme Activation
13) Effect of pH and temperature on enzyme kinetics
14) Irreversible inactivation kinetics of enzyme.
15) repeat
16) exam
Recommended or Required Reading
1- I. H. Segel, Enzyme Kinetics, John-Wiley & Sons, USA, 1975
Planned Learning Activities and Teaching Methods
1) Lecture
2) Question-Answer
3) Discussion
4) Drill and Practice
5) Problem Solving
Assessment Methods and Criteria
Contribution of Midterm Examination to Course Grade |
40% |
|---|---|
Contribution of Final Examination to Course Grade |
60% |
Total |
100% |
Language of Instruction
Turkish
Work Placement(s)
Not Required