Kocaeli University / European Credit Transfer System (ECTS)

Name of Lecturer(s)

Prof. Dr. Yonca DUMAN
Prof. Dr. Neslihan ŞAKİ
Associate Prof. Dr. Mustafa Oğuzhan KAYA

Learning Outcomes of the Course Unit
Program Competencies-Learning Outcomes Relation

Learning Outcomes of the Course Unit

1) Making planning and strategy in enzyme purification.
2) Doing cell disintegration and extraction of intracellular enzymes,
3) Making enzyme purification with various chromatographic and electrophoretic methods.
4) Comment on some applications for enzyme purifications.
5) Discuss application areas of purified enzymes.

Program Competencies-Learning Outcomes Relation

		Program Competencies
		1	2	3	4	5	6
Learning Outcomes
	1	High	High	High	High	High	High
	2	High	High	High	High	High	High
	3	High	High	High	High	High	High
	4	High	High	High	High	High	High
	5	High	High	High	High	High	High

Mode of Delivery

Face to Face

Prerequisites and Co-Requisites

None

Recommended Optional Programme Components

Enzymology

Course Contents

This course provides candidates with profound knowledge on planning and strategy in enzyme purification, methods for cell disintegration, methods for extraction of intracellular enzymes, methods for concentrations of enzyme extracts, principles of enzyme purification based on structural properties, adsorbtion techniques used on enzyme purification, ion exchange and hydrophobic interaction chromatographies, enzyme purification by affinity techniques, enzyme purification based on molecular size, other chromatographic techniques used in enzyme purification, electrophoretic methods in enzyme purification, establishment of enzyme purification profiles, and some applications for enzyme purifications.

Weekly Schedule

1) Planning and strategy in enzyme purification
2) Methods for cell disintegration
3) Methods for extraction of intracellular enzymes
4) Methods for fractionel precipitationof enzyme extracts,
5) Principles of enzyme purification based on structural properties
6) Principles of enzyme purification based on structural properties
7) Adsorbtion techniques used on enzyme purification
8) Midterm examination/Assessment
9) Ion exchange and hydrophobic interaction chromatographies
10) Enzyme purification by affinity techniques
11) Enzyme purification based on molecular size
12) Other chromatographic techniques used in enzyme purification
13) Electrophoretic methods in enzyme purification
14) Establishment of enzyme purification profiles
15) Some applications for enzyme purifications
16) Final examination

Planned Learning Activities and Teaching Methods

1) Lecture
2) Discussion
3) Demonstration
4) Group Study
5) Problem Solving

Assessment Methods and Criteria

Contribution of Midterm Examination to Course Grade	40%
Contribution of Final Examination to Course Grade	60%
Total	100%

Language of Instruction

Turkish

Work Placement(s)

Not Required

Course Unit Title	Course Unit Code	Type of Course Unit	Level of Course Unit	Year of Study	Semester	ECTS Credits
Basic Methods In Enzyme Purification	KIM507	Elective	Master's degree	1	Fall	8