Molecular Biology Methods II
Biology
Institute of Natural and Applied Sciences
Second Cycle (Master's Degree)
| Course Unit Title | Course Unit Code | Type of Course Unit | Level of Course Unit | Year of Study | Semester | ECTS Credits |
|---|---|---|---|---|---|---|
| Molecular Biology Methods II | BIO539 | Elective | Master's degree | 1 | Spring | 8 |
Name of Lecturer(s)
Prof. Dr. Fikriye POLAT
Associate Prof. Dr. Yonca YÜZÜGÜLLÜ KARAKUŞ
Learning Outcomes of the Course Unit
1) Explaining Genomic DNA isolation.
2) Learning to do Primer DNA design.
3) Amplify a gene using PCR.
4) Learning which electrophoresis techniques are used for researches based on DNA and protein.
Program Competencies-Learning Outcomes Relation
| Program Competencies | ||||||||||||||||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | ||
| Learning Outcomes | ||||||||||||||||||||||||||||||
| 1 | High | No relation | High | High | High | High | No relation | No relation | No relation | No relation | High | Middle | No relation | No relation | No relation | No relation | High | No relation | No relation | No relation | No relation | No relation | No relation | No relation | No relation | High | Middle | High | No relation | |
| 2 | High | No relation | High | No relation | High | High | No relation | No relation | No relation | No relation | High | Middle | No relation | No relation | No relation | No relation | High | No relation | No relation | No relation | No relation | No relation | No relation | No relation | No relation | High | Middle | High | No relation | |
| 3 | Middle | No relation | High | No relation | High | No relation | No relation | No relation | No relation | No relation | High | Middle | No relation | No relation | No relation | No relation | High | No relation | No relation | No relation | No relation | No relation | No relation | No relation | No relation | No relation | No relation | No relation | No relation | |
| 4 | Middle | No relation | High | No relation | High | No relation | No relation | No relation | No relation | No relation | High | Middle | No relation | No relation | No relation | No relation | High | No relation | No relation | No relation | No relation | No relation | No relation | No relation | No relation | High | Middle | High | No relation | |
Mode of Delivery
Face to Face
Prerequisites and Co-Requisites
None
Recommended Optional Programme Components
n/a
Course Contents
Primer design, PCR, Electrophoreses techniques (Agarose gel electrophoreses, Poliacrylamid gel electrophoreses, Isoelectric focusing, Two dimensional electrophoreses), techniques after electrophoreses ( Dyeing, Blotting, İsolation) Sequence Analysis, Proteomics
Weekly Schedule
1) Primer design2) Primer design (continue)
3) PCR
4) PCR
5) Agarose gel electrophoreses
6) Agarose gel electrophoreses
7) Poliacrylamid gel electrophoreses
8) Midterm examination/Assessment
9) Poliacrylamid gel electrophoreses
10) Poliacrylamid gel electrophoreses
11) Isoelectric focusing
12) Two dimensional electrophoreses
13) Techniques after electrophoreses ( Dyeing, Blotting, İsolation)
14) Sequence Analysis
15) Proteomics
16) Final examination
Recommended or Required Reading
1- Moleküler Biyoloji- Ahmet YILDIRIM, Fevzi BARDAKÇI
2- Gen Klonlama ve DNA Anazlizi-Fevzi BARDAKÇI, Ali Fazıl YENİDÜNYA, Nazan YILMAZ
3- İnternette ilgili web siteleri
4- Makaleler
Planned Learning Activities and Teaching Methods
1) Lecture
2) Discussion
3) Demonstration
4) Group Study
5) Problem Solving
Assessment Methods and Criteria
Contribution of Midterm Examination to Course Grade |
40% |
|---|---|
Contribution of Final Examination to Course Grade |
60% |
Total |
100% |
Language of Instruction
Turkish
Work Placement(s)
Not Required