Plant Tissue Culture Applications

Mode of Delivery

Face to Face

Prerequisites and Co-Requisites

None

Recommended Optional Programme Components

Not Required

Course Contents

Plant tissue culture laboratory and devices used, related chemicals, areas of use of the technique, and current applications are explained. Plant tissue culture techniques are applied using various organs of sample plant species, and the effects of plant growth regulators on plants are demonstrated comparatively in practice.

Weekly Schedule

1) Totipotency, cell division and elongation in plants
2) History of plant tissue culture and current application purposes of the technique
3) Introducing laboratory parts and teaching the use of related devices
4) Plant growth regulators, working concentrations and storage conditions
5) Aseptic techniques and sterilization practices
6) Medium components; macro and micro elements, other organic substances and buffers
7) Bitkiye uygun besiyerinin seçilmesi, hazırlanması ve sterilizasyonu
8) Midterm Exam
9) Selection and optimization of a disinfection method
10) In vitro germination of seeds and obtaining explants
11) Establishment of cultures using apical or lateral meristems, and shoot multiplication
12) Establishment of callus cultures from leaf explants and properties of callus tissue
13) Establishment of cell suspension cultures and calculation of cell density
14) Introduction of synthetic seed production stages
15) Quantitative and qualitative evaluation of culture data
16) Final Exam

Recommended or Required Reading

1- George, E. F., Hall, M. A., & Klerk, G. D. (2008). Plant propagation by tissue culture: Volume 1. The background. Springer.
2- Leva, A., & Rinaldi, L. (2012). Recent advances in plant in vitro culture. InTech Open.
3- Lumsden, P. J., Nicholas, J. R., Davies, W. J. (1994). Physiology, growth and development of plants in culture. Springer.

Planned Learning Activities and Teaching Methods

1) Lecture
2) Lecture
3) Lecture
4) Question-Answer
5) Question-Answer
6) Question-Answer
7) Question-Answer
8) Discussion
9) Discussion
10) Discussion
11) Discussion

Assessment Methods and Criteria

Contribution of Midterm Examination to Course Grade	40%
Contribution of Final Examination to Course Grade	60%
Total	100%

Language of Instruction

Turkish

Work Placement(s)

Not Required