Molecular Biology

Mode of Delivery

Face to Face

Prerequisites and Co-Requisites

None

Recommended Optional Programme Components

Medical Biology

Course Contents

Lecture titles: 1. Biochemical basis of molecular biology The flow of genetic information, Nucleic asid biochemistry, DNA metobolizing enzymes, Biochemical techniques to study DNA and RNA (Purification of nucleic acids, Gel electrophoresis, DNA sequencing, Blotting, DNA sequence analysis techniques). 2. The tools of molecular biology E. coli K12 and its properties, Lac operon and the use of lac operon in molecular biology, bacterial plazmids, Bacteriophage based plasmids. 3. Gene analysis methods Creating a DNA library, screening and analysis. 4. Genome organization E. coli K12 genome S. cerevisiae genome Human genome Genome mapping New generation genome sequencing Genome data analysis and contig assembly Pulse field electrophoresis and analysis of fragmented DNA

Weekly Schedule

1) Cells and Macromolecules
2) Protein Structure
3) Properties of Nucleic Acids
4) Chromosome Structure
5) DNA Replication
6) DNA Damage Repair and Recombination
7) Gene Manipulations
8) Midterm Examination/Assessment
9) Analysis and Uses of Cloned DNA
10) Transcription in Prokaryotes
11) Transcription in Eukaryotes
12) Regulation of Transcription
13) RNA Processing and RNPs
14) The Genetic Code and tRNA
15) Cloning Vectors
16) Final Examination

Recommended or Required Reading

1- ders notları

Planned Learning Activities and Teaching Methods

1) Lecture
2) Question-Answer
3) Discussion
4) Group Study
5) Lab / Workshop

Assessment Methods and Criteria

Contribution of Midterm Examination to Course Grade	40%
Contribution of Final Examination to Course Grade	60%
Total	100%

Language of Instruction

Turkish

Work Placement(s)

Not Required