Molecular Genetic Techniques
Medical Biology
Institute of Health Sciences
Second Cycle (Master's Degree)
| Course Unit Title | Course Unit Code | Type of Course Unit | Level of Course Unit | Year of Study | Semester | ECTS Credits |
|---|---|---|---|---|---|---|
| Molecular Genetic Techniques | TBG508 | Elective | Master's degree | 1 | Spring | 6 |
Name of Lecturer(s)
Prof. Dr. Gürler AKPINAR
Prof. Dr. Murat KASAP
Learning Outcomes of the Course Unit
1) Use molecular techniques to solve scientific problems
2) Design new projects
3) Explain gene organizations in procaryotes and eukaryotes
4) Conduct group work in a team project
5) Conduct scientific research in the field
6) How to perform experiments
Program Competencies-Learning Outcomes Relation
| Program Competencies | ||||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | ||
| Learning Outcomes | ||||||||||||||||
| 1 | Middle | Low | No relation | Middle | No relation | Middle | No relation | No relation | Middle | No relation | No relation | Middle | No relation | No relation | Middle | |
| 2 | Middle | No relation | Middle | No relation | No relation | Middle | No relation | No relation | No relation | Low | Middle | No relation | Middle | No relation | Middle | |
| 3 | Middle | Low | Low | Low | Middle | Low | No relation | Low | Middle | No relation | Low | No relation | Low | Middle | No relation | |
| 4 | Low | Middle | Low | Middle | Middle | Middle | Middle | Middle | High | Low | Middle | Middle | Middle | Middle | Middle | |
| 5 | Low | Middle | Middle | Low | Middle | Middle | Middle | Middle | Middle | Middle | Middle | Middle | Middle | Middle | Middle | |
| 6 | Middle | Middle | Low | Low | Middle | High | Middle | Low | No relation | Middle | Middle | Low | High | Middle | High | |
Mode of Delivery
Face to Face
Prerequisites and Co-Requisites
None
Recommended Optional Programme Components
Literature reading
Course Contents
Biochemical basis of applied molecular genetics Laboratory tools for molecular genetics applications Overview of the gene analysis methods Charecterization of genomic DNA Isolation and characterization of gene transcripts PCR, real time PCR and its applicaitons Expression of cloned genes in cultured cells
Weekly Schedule
1) primer design and ordering2) PCR and GFP amplification
3) Analysis of gene products by gel electrophoresis
4) Digestion of PCR produts
5) Ligation of PCR products
6) Transformation of ligation products
7) Single colony selection
8) Maxi Prep and PLasmid isolation
9) Preparation of protein expression cultures and media
10) E. coli cultures for protein expression
11) SDS-PAGE and verification of protein expression
12) Nİ-NTA chromatography and protein purification
13) Analysis of purified protein
14) Exam Preparation
15) Questions and answers discussion
16) exam week
Recommended or Required Reading
1- Ders notları
2- Ders Kitaba
Planned Learning Activities and Teaching Methods
1) Lecture
2) Lecture
3) Question-Answer
4) Question-Answer
5) Discussion
Assessment Methods and Criteria
Contribution of Midterm Examination to Course Grade |
40% |
|---|---|
Contribution of Final Examination to Course Grade |
60% |
Total |
100% |
Language of Instruction
Turkish
Work Placement(s)
Not Required