Basic Research Mthods In Mlecular Bology
Medical Genetics and Molecular Biology
Institute of Health Sciences
Second Cycle (Master's Degree)
| Course Unit Title | Course Unit Code | Type of Course Unit | Level of Course Unit | Year of Study | Semester | ECTS Credits |
|---|---|---|---|---|---|---|
| Basic Research Mthods In Mlecular Bology | MBG511 | Elective | Master's degree | 1 | Fall | 3 |
Name of Lecturer(s)
Prof. Dr. Naci ÇİNE
Assistant Prof. Dr. Seda EREN KESKİN
Learning Outcomes of the Course Unit
1) Applying molecular biology techniques
2) Being equipped with laboratory protocol knowledge at advanced level.
3) Review historical perspective the development process of the protocols.
4) Defining the practical objectives of molecular biology protocols and explain their preference reasons in aswering to specific questions..
5) Conducting a research using basic research techniques of molecular biology
Program Competencies-Learning Outcomes Relation
| Program Competencies | ||||||
| 1 | 2 | 3 | 4 | 5 | ||
| Learning Outcomes | ||||||
| 1 | No relation | No relation | No relation | No relation | No relation | |
Mode of Delivery
Face to Face
Prerequisites and Co-Requisites
None
Recommended Optional Programme Components
Medical genetics
Course Contents
In this course; plasmids and their usefulness in molecular cloning, bacteriophage and itv Vectors, Gl Electrophoresis of DNA and Pulsed-Field Agarose, Preparation and Analysis of Eukaryotic Genomic DNA, Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells, In Vitro Amplification of DNA by the Polymerase Chain Reaction, working with Synthetic Oligonucleotide Probes, DNA Sequencing, site-directed Mutagenesis, screening expression libraries, expression of Cloned Genes in Escherichia coli, introducing Cloned Genes into Cultured Mammalian Cells, analysis of Gene Expression in Cultured Mammalian Cells and Recombinant protein purification techniques are introdcued.
Weekly Schedule
1) DNA and RNA isolation methods2) Qualitative and quantitative determination of DNA and RNA
3) Principles and types of electrophoresis
4) Blotting techniques
5) Gene transfer
6) Genetic expression
7) In vitro mutagenesis
8) Midterm examination/Assessment
9) Literature review
10) Literature review
11) Literature review
12) Literature review
13) Literature review
14) Literature review
15) Literature review
16) Final examination
Recommended or Required Reading
1- Moleküler Biyolojide Kullanılan Yöntemler, 2. Baskı, Temizkan G., Arda N., Nobel Tıp Kitabevleri, 2004
2- Thompson&Thompson Tıbbi Genetik (Thompson&Thompson Genetics in Medicine)
3- Tıbbi Genetik / Nurettin Başaran
4- Genetics in Practice / Jo Hayden
5- The Genetic Family History / Robin L. Bennett
6- Moleküler Biyolojide Kullanılan Yöntemler, 2. Baskı, Temizkan G., Arda N., Nobel Tıp Kitabevleri, 2004
7- Moleküler Biyolojide Kullanılan Yöntemler, 2. Baskı, Temizkan G., Arda N., Nobel Tıp Kitabevleri, 2004
8- Moleküler Biyolojide Kullanılan Yöntemler, 2. Baskı, Temizkan G., Arda N., Nobel Tıp Kitabevleri, 2004
9- Moleküler Biyolojide Kullanılan Yöntemler, 2. Baskı, Temizkan G., Arda N., Nobel Tıp Kitabevleri, 2004
10- Moleküler Biyolojide Kullanılan Yöntemler, 2. Baskı, Temizkan G., Arda N., Nobel Tıp Kitabevleri, 2004
11- Moleküler Biyolojide Kullanılan Yöntemler, 2. Baskı, Temizkan G., Arda N., Nobel Tıp Kitabevleri, 2004
Planned Learning Activities and Teaching Methods
1) Lecture
2) Question-Answer
3) Discussion
4) Group Study
5) Lab / Workshop
Assessment Methods and Criteria
Contribution of Semester Studies to Course Grade |
40% |
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Contribution of Final Examination to Course Grade |
60% |
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Total | 100% | |||||||||||
Language of Instruction
Turkish
Work Placement(s)
Not Required