Gene Molecular Biology

Mode of Delivery

Face to Face

Prerequisites and Co-Requisites

None

Recommended Optional Programme Components

Medical genetics

Course Contents

The properties of nucleic acid molecules that form the basis of genetic information of organisms, and packaging of prokaryotic and eukaryotic organisms to understand the organization of chromosomes, DNA replication, transcription and translation processes such as proliferation and gene expression that are essential to understand and be able to compare how the prokaryotic and eukaryotic organisms, the basics of genetic code are among the scopes of this course.

Weekly Schedule

1) DNA Structure and Analysis
2) Genetics Material Properties
3) RNA and proteins in which the macromolecule to be used in the methods of analysis of solutions and buffers required is performed.
4) RNA isolation and analysis methods, types of macromolecules in plants using Trizol RNA isolation and spectral analysis of RNA isolated from
5) RNA isolation and analysis methods, types of macromolecules, RNAs isolated from mammalian cells and RNA isolation using the spectral analysis of TriPure
6) Electrophoretic analysis of RNA macromolecules, RNA electrophoretic analysis of nucleic acid hybridization techniques (Northern blotting, Southern Blotting, FISH, ISH) provide theoretical information, plant and mammalian isolates, electrophoretic analysis of total RNA isolated from cells (MOPS gel electrophoresis), gel electrophoresis and comparison of the results reported and evaluated and presented as spectrophotometry
7) Macromolecule Processing and quantification of RNA, cDNA synthesis RNA'lardan be isolated from mammalian cells, cDNA spectral analysis of the cDNA by PCR with primers of different genomic region reproduction of the results by agarose gel electrophoresis to display the results of the comparison of genomic and cDNA PCR
8) Midterm examination/Assessment
9) Methods of isolation and quantification of proteins
10) Methods of isolation and quantification of proteins in plants with a total protein extraction and quantification method of Bradford
11) Methods of isolation and quantification of proteins, mammalian cells, total protein isolation, and Bradford, Lowry methods of quantification, as the submission of a report evaluating the results
12) Protein electrophoresis methods, SDS-PAGE, gel staining methods, isoelectric focusing, 2D gel electrophoresis and gel drying methods taught in theory, the execution of the isolated proteins by SDS-PAGE gel electrophoresis and Coomassie staining and presented as a report evaluating the results
13) Methods of concentrating proteins, the general principles of chromatography, to isolate the cell components of the protein, cytoplasmic and mitochondrial protein isolated from mammalian cells, quantification by Bradford
14) Analysis of proteins as macromolecules, molecular biological techniques, ELISA method, in theory, working principle and types of teaching, learning to estimate protein concentration by ELISA in serum samples, giving results as a report, by the course of molecular biology techniques to investigate their use in the study of molecular biology techniques are committed student research presentations
15) Presentation
16) Final examination

Recommended or Required Reading

1- Molecular Biology of the Gene, Fifth Edition, James D Watson
2- Thompson&Thompson Tıbbi Genetik (Thompson&Thompson Genetics in Medicine)
3- Hücre Moleküler Yaklaşım, Üçüncü Baskı / Geoffey M. Cooper, Robert E. Haulman
4- Moleküler Genetiğin Esasları / Doç. Dr. H. Ümit Lüleyap
5- Tıbbi Genetik / Başaran N. , Nobel Tıp Kİtabevi, Eskişehir, 2003
6- Molecular Biology of the Gene, Fifth Edition, James D Watson
7- Molecular Biology of the Gene, Fifth Edition, James D Watson
8- Molecular Biology of the Gene, Fifth Edition, James D Watson
9- Molecular Biology of the Gene, Fifth Edition, James D Watson
10- bir ara,bir final
11- an intermediate, a final

Planned Learning Activities and Teaching Methods

1) Lecture
2) Question-Answer
3) Discussion
4) Group Study
5) Lab / Workshop

Assessment Methods and Criteria

Contribution of Semester Studies to Course Grade	40%
Number Percentage Semester Studies Midterm Examination 1 60% Practices 1 40%
Contribution of Final Examination to Course Grade	60%
Total	100%

Language of Instruction

Turkish

Work Placement(s)

Not Required