Aseptic Cell Culture Techniques (for Master Students)

Mode of Delivery

Face to Face

Prerequisites and Co-Requisites

None

Recommended Optional Programme Components

Not Required

Course Contents

The isolation and characterization of human and animal cells and tissues for culture, in vitro culture (disease and tumor cell culture models); benefits and limitations of cell and tissue cultures; laboratory planning and use; aseptic conditions; biosafety and quality assurance; good production / laboratory / cell culture / stem cell culture conditions, primer culture and passaging methods; cell noun; Contamination; cytotoxicity; specialized cells; culture methods with and without nutrient layer.

Weekly Schedule

1) Basic cell culture techniques introduction
2) Biology of cells in culture
3) Laboratory layout and equipment
4) Aseptic technique, safety, bioethics and validation
5) Culture containers, culture conditions, subculturing
6) Identified Media formulations
7) Serum-free media, preparation and sterilization
8) midterm
9) Primary culture and cell lines
10) Cloning and cell selection
11) Cell characterization
12) Transformastion ve contamination
13) Criyopreservation
14) Viability and proliferation tests of cells in culture, cytotoxicity
15) Culture of specific cells and tumor cells
16) Final exam

Recommended or Required Reading

1- Culture of Human Stem Cells.R. Ian Freshney, Glyn N. Stacey, Jonathan M. Auerbach. 2007, Wiley-Blackwell ISBN: 978-0-470-05246-4
2- Stem cells: scientific progress and future research directions. National Institutes of Health. November 2004, University Press of the Pacific. ISBN: 978-1-4102-1896-4

Planned Learning Activities and Teaching Methods

1) Lecture
2) Question-Answer
3) Discussion
4) Demonstration
5) Lab / Workshop
6) Self Study

Assessment Methods and Criteria

Contribution of Semester Studies to Course Grade	40%
Number Percentage Semester Studies Midterm Examination 1 40% Laboratory 2 30% Presentation/Seminar 2 30%
Contribution of Final Examination to Course Grade	60%
Total	100%

Language of Instruction

Turkish

Work Placement(s)

Not Required