Stem Cell Staining Techniques(for Master Students)
Stem Cell and Tissue Regeneration
Institute of Health Sciences
Second Cycle (Master's Degree)
| Course Unit Title | Course Unit Code | Type of Course Unit | Level of Course Unit | Year of Study | Semester | ECTS Credits |
|---|---|---|---|---|---|---|
| Stem Cell Staining Techniques(for Master Students) | KHT527 | Elective | Master's degree | 1 | Spring | 7 |
Name of Lecturer(s)
Prof. Dr. Yusufhan YAZIR
Associate Prof. Dr. Gökhan DURUKSU
Learning Outcomes of the Course Unit
1) Have knowledge about culture techniques of stem cells and methods of in vivo application.
2) Basic principles of painting and techniques used in painting, have information about materials.
3) Have knowledge about immunohistochemical and immunofluorescent staining used in stem cell studies.
4) Texture follow-up, will have information about the problems and solutions during painting.
Program Competencies-Learning Outcomes Relation
| Program Competencies | |||||||
| 1 | 2 | 3 | 4 | 5 | 6 | ||
| Learning Outcomes | |||||||
| 1 | No relation | No relation | No relation | No relation | No relation | No relation | |
| 2 | No relation | No relation | No relation | No relation | No relation | No relation | |
| 3 | No relation | No relation | No relation | No relation | No relation | No relation | |
| 4 | No relation | No relation | No relation | No relation | No relation | No relation | |
Mode of Delivery
Face to Face
Prerequisites and Co-Requisites
None
Recommended Optional Programme Components
Not Required
Course Contents
Methods to be followed in the step-by-step staining of stem cells, alternative stains and staining methods, problems that may occur during these operations and ways of coping with them
Weekly Schedule
1) Stem cells: cell culture techniques2) In vivo application methods of stem cells
3) Basic principles of staining
4) Fixation techniques for cells for staining
5) Buffer solution preparation
6) Immunohistochemical stains used in stem cell studies
7) Immunofluorescent stains used in stem cell studies
8) Mıdterm
9) Immunofluorescent staining method for cells in cell culture
10) What is primer and secondary antibody?
11) What to consider when choosing primary and secondary antibodies
12) What is tissue tracking and how to do it?
13) Ways to be followed in stains after tissue processing
14) What is double staining and how is it done?
15) Problems that may be encountered during staining and their solutions
16) Final exam
Recommended or Required Reading
1- Kök Hücre. Prof.Dr. Alp CAN, 2013. Akademisyen Kitabevi, ISBN:9786054649433
2- Yaşam Bilimlerinde A’dan Z’ye Mikroskopi, Prof. Dr. Alp CAN, Nobel Tıp Yayınevi, 2018, ISBN: 9786059215664
Planned Learning Activities and Teaching Methods
1) Lecture
2) Question-Answer
3) Discussion
4) Lab / Workshop
Assessment Methods and Criteria
Contribution of Midterm Examination to Course Grade |
40% |
|---|---|
Contribution of Final Examination to Course Grade |
60% |
Total |
100% |
Language of Instruction
Turkish
Work Placement(s)
Not Required