Recombinant Dna Technologies
Stem Cell and Tissue Regeneration
Institute of Health Sciences
Second Cycle (Master's Degree)
| Course Unit Title | Course Unit Code | Type of Course Unit | Level of Course Unit | Year of Study | Semester | ECTS Credits |
|---|---|---|---|---|---|---|
| Recombinant Dna Technologies | KHT531 | Elective | Master's degree | 1 | Spring | 7 |
Name of Lecturer(s)
Associate Prof. Dr. Gökhan DURUKSU
Learning Outcomes of the Course Unit
1) Recognizing basic concepts of recombinant DNA technology
2) Using the recombinant DNA technology in the directional differentiation of stem cells
3) Using recombinant DNA technology methods in the applications of tissue regeneration and tissue engineering
4) Evaluating the importance of recombinant DNA technology in stem cell biology and applications with a critical view
5) Gaining critical view on different approaches of gene therapies
Program Competencies-Learning Outcomes Relation
| Program Competencies | |||||||
| 1 | 2 | 3 | 4 | 5 | 6 | ||
| Learning Outcomes | |||||||
| 1 | No relation | No relation | No relation | No relation | No relation | No relation | |
| 2 | No relation | No relation | No relation | No relation | No relation | No relation | |
| 3 | No relation | No relation | No relation | No relation | No relation | No relation | |
| 4 | No relation | No relation | No relation | No relation | No relation | No relation | |
| 5 | No relation | No relation | No relation | No relation | No relation | No relation | |
Mode of Delivery
Face to Face
Prerequisites and Co-Requisites
None
Recommended Optional Programme Components
Not Required
Course Contents
The relation among gene, peptide and protein, modification of DNA and its tools, fundamental methods in recombinant DNA technology, regulation of gene expression, viruses and gene transfer, gene modification in stem cells, cell programming and differentiation, epigenetic and recombination are among the scopes of this course.
Weekly Schedule
1) The relation between gene, peptide and protein.2) Control of gene expression.
3) Modification of DNA and its tools.
4) Fundamental methods in recombinant DNA technology.
5) Recombinant DNA technology
6) Regulation of gene expression.
7) Viruses and gene transfer.
8) Midterm examination/Assessment
9) Gene modification in stem cells.
10) Gene modification in stem cells.
11) Gene modification in stem cells.
12) Cell programming and differentiation.
13) Epigenetic and recombination.
14) Ethical aspects of recombination
15) Newly developed techniques and approaches
16) Final examination
Recommended or Required Reading
1- Molecular Cloning: A Laboratory Manual, 3th Edition.Joseph Sambrook, David W. Russell. January 2001, Cold Spring Harbor Pub. ISBN 978-0-8796-9577-4
2- Principles of Gene Manipulation and Genomics, 7th Edition. Sandy B. Primrose, Richard Twyman. January 2006, Wiley-Blackwell. ISBN 978-1-4051-3544-3
Planned Learning Activities and Teaching Methods
1) Lecture
2) Question-Answer
3) Project Based Learning
Assessment Methods and Criteria
Contribution of Semester Studies to Course Grade |
50% |
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Contribution of Final Examination to Course Grade |
50% |
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Total | 100% | |||||||||||
Language of Instruction
Turkish
Work Placement(s)
Not Required