Laboratory and Characterization Techniques In Stem Cells
Stem Cell and Tissue Regeneration
Institute of Health Sciences
Second Cycle (Master's Degree)
| Course Unit Title | Course Unit Code | Type of Course Unit | Level of Course Unit | Year of Study | Semester | ECTS Credits |
|---|---|---|---|---|---|---|
| Laboratory and Characterization Techniques In Stem Cells | KHT536 | Compulsory | Master's degree | 1 | Spring | 7 |
Name of Lecturer(s)
Associate Prof. Dr. Gökhan DURUKSU
Learning Outcomes of the Course Unit
1) To gain the knowledge and the practice to charaterize the stem cells
Program Competencies-Learning Outcomes Relation
| Program Competencies | |||||||
| 1 | 2 | 3 | 4 | 5 | 6 | ||
| Learning Outcomes | |||||||
| 1 | No relation | No relation | No relation | No relation | No relation | No relation | |
Mode of Delivery
Face to Face
Prerequisites and Co-Requisites
None
Recommended Optional Programme Components
Not Required
Course Contents
Stem cell isolation methods, definition and application of culture and differentiation methods, determination of cell viability and measurement of proliferation, methods for characterization of stem cells
Weekly Schedule
1) Basic concepts of cell culture2) Microbial and chemical contaminants
3) Cell culture laboratories
4) Isolation and culture of mesenchymal stem cells
5) Isolation and culture of embryonic stem cells
6) Isolation and culture of other stem cells
7) Basic mistakes in cell culture
8) Mid-Term
9) Stem cell differentiation and characterization
10) Stem cell differentiation and characterization
11) Cell aging and characterization
12) Apoptosis, necrosis and autophagy
13) GLP, GHP and GMP applications
14) Stem cell production under GMP conditions
15) ...
16) Final Exam
Recommended or Required Reading
1- Stem Cell Biology: A Practical Laboratory Manual. Dr. Indumathi Somasundaram, Dr. Dhanasekaran Marappagounder, Dr. Pankaj Kaingade. Evincepub Publishing , 2020. ISBN:9389774357.
Planned Learning Activities and Teaching Methods
1) Lecture
2) Question-Answer
3) Drill and Practice
4) Lab / Workshop
5) Self Study
Assessment Methods and Criteria
Contribution of Semester Studies to Course Grade |
60% |
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Contribution of Final Examination to Course Grade |
40% |
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Total | 100% | |||||||||||
Language of Instruction
Turkish
Work Placement(s)
Not Required