Molecular Biological Techniques
Molecular Gastroenterology and Hepatology
Institute of Gastroenterology and Hepatology
Second Cycle (Master's Degree)
| Course Unit Title | Course Unit Code | Type of Course Unit | Level of Course Unit | Year of Study | Semester | ECTS Credits |
|---|---|---|---|---|---|---|
| Molecular Biological Techniques | GHE108 | Elective | Master's degree | 1 | Spring | 6 |
Name of Lecturer(s)
Assistant Prof. Dr. Doğanhan Kadir ER
Learning Outcomes of the Course Unit
1) Learns the mechanisms of molecular biological tests.
2) When they read the articles, they can comprehend molecular biological methods.
3) He/she can choose the appropriate molecular technique for his/her planned study.
4) Identify potential errors that may occur during the application of the molecular technique.
Program Competencies-Learning Outcomes Relation
| Program Competencies | ||||||
| 1 | 2 | 3 | 4 | 5 | ||
| Learning Outcomes | ||||||
| 1 | Middle | Middle | High | Middle | High | |
| 2 | Middle | High | High | Middle | High | |
| 3 | Middle | Middle | High | Middle | High | |
| 4 | Middle | High | High | Middle | High | |
Mode of Delivery
Face to Face
Prerequisites and Co-Requisites
None
Recommended Optional Programme Components
Not Required
Course Contents
Within the scope of this course, basic molecular techniques will be investigated to help students develop their thesis projects. These techniques include PCR, RT-PCR, DNA, RNA and Protein gel electrophoresis, gene cloning, protein expression and purification.
Weekly Schedule
1) Biosafety2) Nucleic acid isolation techniques
3) Polymerase chain reaction and types of PCR - I
4) Polymerase chain reaction and types of PCR - II
5) Agarose gel electrophoresis
6) Molecular typing methods - I
7) Midterm Exam
8) Molecular typing methods - II
9) Molecular typing methods - III
10) Sequencing techniques and next-generation sequencing
11) Blotting techniques - I
12) Blotting techniques - II
13) Cell culture and applications of cell culture
14) Microarray technology
15) Syndromic testing
16) Final Exam
Recommended or Required Reading
1- Methods in molecular biology
2- Basic methods in molecular biology
3- Ders notları
Planned Learning Activities and Teaching Methods
1) Lecture
2) Question-Answer
3) Discussion
4) Self Study
Assessment Methods and Criteria
Contribution of Midterm Examination to Course Grade |
30% |
|---|---|
Contribution of Final Examination to Course Grade |
70% |
Total |
100% |
Language of Instruction
Turkish
Work Placement(s)
Not Required