Rna and Protein Technologies

Mode of Delivery

Face to Face

Prerequisites and Co-Requisites

None

Recommended Optional Programme Components

Medical genetics

Course Contents

Classical protein isolation methods such as ion exchange chromatography, gel filtration, hydrophobic interactions, hydroxy apitite and as well as the methods developed after the onset of recombinant DNA technology such as Ni-NTA purification, S-TAG purification and Mal-Tag purification. Proteomics technology and how it is being used in identification of protein markers.

Weekly Schedule

1) The protein technology
2) RNA TECHNOLOGY
3) PROTEIN STRUCTURE
4) Enzyme Reactions
5) KINETICS OF PROTEIN
6) Protein Purification Methods
7) NORTHERN & WESTERN BLOTTİNG
8) Midterm examination/Assessment
9) APPLICATION METHOD RNA AND Protein Microarray
10) Use of RNA Molecule Gene Expression
11) REAL TIME PCR
12) UNDERSTANDING THE PROCESS Proteomics
13) APPLICATION PROCESS PROTEOMICS
14) RNA TECHNOLOGY
15) PROTEIN TECHNOLOGY
16) Final examination

Recommended or Required Reading

Planned Learning Activities and Teaching Methods

1) Lecture
2) Question-Answer
3) Discussion
4) Group Study
5) Lab / Workshop

Assessment Methods and Criteria

Contribution of Midterm Examination to Course Grade	70%
Contribution of Final Examination to Course Grade	30%
Total	100%

Language of Instruction

Turkish

Work Placement(s)

Not Required