| Course Unit Title | Course Unit Code | Type of Course Unit | Level of Course Unit | Year of Study | Semester | ECTS Credits |
|---|---|---|---|---|---|---|
| Tissue Culture | ZBB315 | Elective | Bachelor's degree | 3 | Fall | 3 |
Name of Lecturer(s)
Associate Prof. Dr. Melekber SÜLÜŞOĞLU DURUL
Learning Outcomes of the Course Unit
1) Identifies plant tissue culture
2) Knows the tools and equipment used in the tissue culture laboratory
3) Knows the sterile working principles
4) Knows the principles of preparation of nutrient media required for tissue cultures
5) Apply plant tissue culture techniques
6) Knows the techniques and procedures applied in plant tissue culture and compares the results.
7) Understands the usage areas of plant tissue culture
Program Competencies-Learning Outcomes Relation
| Program Competencies | ||||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | ||
| Learning Outcomes | ||||||||||||
| 1 | No relation | No relation | No relation | Middle | High | No relation | No relation | No relation | No relation | No relation | No relation | |
| 2 | No relation | No relation | No relation | No relation | No relation | High | No relation | Middle | No relation | No relation | No relation | |
| 3 | No relation | No relation | No relation | High | No relation | No relation | No relation | Middle | No relation | No relation | No relation | |
| 4 | No relation | No relation | No relation | High | No relation | No relation | No relation | No relation | No relation | No relation | No relation | |
| 5 | No relation | No relation | No relation | High | High | No relation | No relation | No relation | No relation | No relation | No relation | |
| 6 | No relation | No relation | No relation | High | High | No relation | No relation | No relation | No relation | No relation | No relation | |
| 7 | No relation | No relation | No relation | High | High | No relation | No relation | No relation | No relation | No relation | Middle | |
Mode of Delivery
Face to Face
Prerequisites and Co-Requisites
None
Recommended Optional Programme Components
Not Required
Course Contents
This course included; The organization of the tissue culture laboratory, the nutrient components used in tissue culture, preparation of nutrient media components techniques in the tissue culture laboratory, tissue culture sterilization techniques, nutrient medium preparation techniques, embryo, meristem, callus, cell and protoplast culture techniques applied in plants, haploid plant acquisition techniques, herbal It includes topics such as rapid and multiple production of materials, somaclonal variation, somatic hybridization, secondary metabolite production.
Weekly Schedule
1) Definition of tissue culture, organization of the laboratory, introduction of tools and equipment2) Nutrient media, ingredients, chemicals and their properties
3) Solution preparation techniques in tissue culture laboratory
4) Preparation and storage of nutrient medium mineral stock hazırlanması ve muhafazası solutions
5) Sources of infection, reasons and sterilization techniques in tissue culture
6) Preparation of nutrient medium and storage
7) Plant material sterilization, explant isolation and culture in tissue culture
8) midterm exam
9) Application and usage areas of embryo and anther culture
10) Shoot tip and meristem culture
11) Callus, cell and protoplast culture
12) Haploid plant recovery in tissue culture
13) Somaclonal variation and somatic hybridization
14) Protoplast culture and fusion, plant growth
15) Acclimatization in tissue culture, evaluation of results
16) final exam
Recommended or Required Reading
Planned Learning Activities and Teaching Methods
1) Lecture
2) Discussion
3) Drill and Practice
4) Lab / Workshop
Assessment Methods and Criteria
Contribution of Semester Studies to Course Grade |
60% |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
||||||||||||
Contribution of Final Examination to Course Grade |
40% |
|||||||||||
Total | 100% | |||||||||||
Language of Instruction
Turkish
Work Placement(s)
Not Required