Basic Principles of Molecular Microbiology
Microbiology
Institute of Health Sciences
Second Cycle (Master's Degree)
| Course Unit Title | Course Unit Code | Type of Course Unit | Level of Course Unit | Year of Study | Semester | ECTS Credits |
|---|---|---|---|---|---|---|
| Basic Principles of Molecular Microbiology | MKB101 | Elective | Master's degree | 1 | Fall | 5 |
Name of Lecturer(s)
Prof. Dr. Aynur KARADENİZLİ
Prof. Dr. Fetiye KOLAYLI
Learning Outcomes of the Course Unit
1) To know DNA and RNA structure in Procaryotes
2) To know PCR principles and prosedurse
3) To know DNA/RNA isolation methods in procaryotes
4) To know Application of PCR in the diagnosis of Infectious Disease
5) To know Various PCR techniques
6) To know In Situ Hibridization Methods
7) To know Typing Methods in Molecular Mİcrobiology
8) To know Cloning procedure
Program Competencies-Learning Outcomes Relation
| Program Competencies | ||||||
| 1 | 2 | 3 | 4 | 5 | ||
| Learning Outcomes | ||||||
| 1 | High | Middle | Middle | Middle | Middle | |
| 2 | High | Middle | High | Middle | Middle | |
| 3 | High | Middle | Middle | Middle | Middle | |
| 4 | High | Low | High | Middle | Middle | |
| 5 | Low | Low | Low | Low | Low | |
| 6 | Middle | Middle | Low | Low | Low | |
| 7 | Middle | Middle | Middle | Middle | Middle | |
| 8 | Middle | Middle | Middle | Middle | Middle | |
Mode of Delivery
Face to Face
Prerequisites and Co-Requisites
None
Recommended Optional Programme Components
.
Course Contents
Contents: 1.History of molecular methods 2.Polymerase Chain Reaction (PCR) • Principle of amplification • Application of PCR in microbiology • Extraction of DNA/RNA • Reaction mix components • Thermocycling • Contamination • Amplicon detection (fragment size analysis) for conventional PCR • DNA gel stains • Optimization procedures in PCR • Limitations of PCR Inhibitors 3. Interpretation of PCR results 4. Real Time PCR methods 5. Typing methods in Molecular Microbiology • Polyacrylamide gel electrophoresis (PAGE) • PCR-based methods (AP-PCR, Rep-PCR, MVLA, AFLP, PCR-RFLP) • PFGE • Sequencing 6. Molecular methods--other than PCR amplification • Signal amplification techniques • Target amplification systems (other than PCR) • Transcription amplification methods • Strand displacement amplification (SDA) • Probe amplification techniques
Weekly Schedule
1) DNA and RNA in procaryotes2) Polymerase chain reaction and its use in microbiology
3) Polymerase chain reaction and its use in microbiology
4) Polymerase chain reaction and its use in microbiology
5) Polymerase chain reaction and its use in microbiology
6) Perform the method of PCR in the diagnosis of infectious disease
7) PCR based techniques
8) Midterm examination/Assessment
9) In-situ Hiibridization methods
10) Typing methods in molecular microbiology
11) Typing methods in molecular microbiology
12) Typing methods in molecular microbiology
13) Contamination in PCR laboratory
14) Molecular detection of nosocomial pathogens
15) Advantages and disadvantages of the molecular methods
16) Final Exam
Recommended or Required Reading
1- Koneman’s Color Atlas and Textbook of Diagnostic Microbiology
Planned Learning Activities and Teaching Methods
1) Lecture
2) Question-Answer
3) Lab / Workshop
Assessment Methods and Criteria
Contribution of Midterm Examination to Course Grade |
30% |
|---|---|
Contribution of Final Examination to Course Grade |
70% |
Total |
100% |
Language of Instruction
Turkish
Work Placement(s)
Not Required