Molecular Genetics I

Mode of Delivery

Face to Face

Prerequisites and Co-Requisites

None

Recommended Optional Programme Components

molecular biolgy

Course Contents

This course covers, DNA structure and gene expression: building blocks and chemical bonds in DNA, RNA and polypeptides, DNA structure and replication, RNA transcription and gene expression, RNA processing, translation, post-translational modifications and protein structure. Chromosome structure and function: structure and function of chromosomes, mitosis and meiosis, cell cycle, studying human chromosomes, chromosome abnormalities. Cells and development: structure and diversity of cells, cell interactions, the specialization of cells during development, pattern formation in development, morphogenesis, early human development: fertilization to gastrulation, neuronal development, conservation of developmental pathways. Genes in pedigrees and populations: monogenic versus multifactorial inheritance, Mendelian pedigree patterns, complications to the basic Mendelian pedigree patterns, Genetics of multifactorial characters, factor affecting gene frequencies. Amplifying DNA: PCR and cell-based DNA cloning: The importance of DNA cloning, PCR: basic futures and applications, principles of cell-based DNA cloning, cloning systems for amplifying different sized fragments, cloning systems for producing single-stranded and mutagenized DNA, cloning systems designed to Express genes. Nucleic acid hybridization: principles and applications: preparation of nucleic acid probes, principles of nucleic acid hybridization, hybridization assays using cloned target DNA and microarrays, Analyzing DNA and gene structure, variation and expression: sequencing and genotyping DNA, identifying genes in cloned DNA and establishing their structure, studying gene expression.

Weekly Schedule

1) DNA replication I
2) DNA replication II
3) DNA structure function relationship
4) RNA structure function relationship I
5) protein structure function relationship
6) genome organization
7) mutations I
8) Mutations II
9) blotting methods
10) human chromosomes overview I
11) Human chromosomes overview II
12) SNPs
13) DNA interacting proteins
14) Overview of the lecture
15) Preperation to exam
16) exam week

Recommended or Required Reading

1- Ders Notları

Planned Learning Activities and Teaching Methods

1) Lecture
2) Lecture
3) Lecture
4) Lecture
5) Lecture
6) Lecture
7) Question-Answer
8) Question-Answer
9) Question-Answer
10) Question-Answer
11) Question-Answer
12) Discussion
13) Discussion
14) Discussion
15) Discussion
16) Discussion
17) Discussion
18) Group Study
19) Group Study
20) Group Study
21) Group Study
22) Group Study
23) Group Study
24) Brain Storming
25) Brain Storming
26) Brain Storming
27) Brain Storming
28) Brain Storming
29) Brain Storming

Assessment Methods and Criteria

Contribution of Midterm Examination to Course Grade	40%
Contribution of Final Examination to Course Grade	60%
Total	100%

Language of Instruction

Turkish

Work Placement(s)

Not Required