Basic Research Methods On Molecular Biology

Mode of Delivery

Face to Face

Prerequisites and Co-Requisites

None

Recommended Optional Programme Components

Medical Genetics

Course Contents

There will be an overview of the methods of analysing genes and their transcripts, characterization of genomic DNA, PCR and its applications, expression of the cloned genes and creation of transgenic multicellular organisms.

Weekly Schedule

1) RNA and protein analysis methods, the macromolecule used in the preparation of buffers and solutions needed to
2) RNA isolation and analysis methods, types of macromolecules in plants using Trizol RNA isolation and spectral analysis of RNA isolated from
3) RNA isolation and analysis methods, types of macromolecules, RNAs isolated from mammalian cells and RNA isolation using the spectral analysis of TriPure
4) Electrophoretic analysis of RNA macromolecules, RNA electrophoretic analysis of nucleic acid hybridization techniques (Northern blotting, Southern Blotting, FISH, ISH) provide theoretical information, plant and mammalian isolates, electrophoretic analysis of total RNA isolated from cells (MOPS gel electrophoresis), gel electrophoresis and comparison of the results reported and evaluated and presented as spectrophotometry
5) Macromolecule Processing and quantification of RNA, cDNA synthesis RNA'lardan be isolated from mammalian cells, cDNA spectral analysis of the cDNA by PCR with primers of different genomic region reproduction of the results by agarose gel electrophoresis to display the results of the comparison of genomic and cDNA PCR
6) RNA processing and quantification of macromolecules; Quantitative PCR analysis methods; Mammalian quantitative PCR using cDNA methods, assessing and reporting the results of quantitative PCR are provided as
7) Methods of isolation and quantification of proteins in plants with a total protein extraction and quantification method of Bradford
8) Methods of isolation and quantification of proteins, mammalian cells, total protein isolation, and Bradford, Lowry methods of quantification, as the submission of a report evaluating the results
9) Analysis of proteins as macromolecules, molecular biological techniques, Western blotting, cytoplasmic and mitochondrial protein extracts isolated from mammalian cells using the SDS-PAGE for Western blotting and elektroblotlama execution
10) Protein electrophoresis methods, SDS-PAGE, gel staining methods, isoelectric focusing, 2D gel electrophoresis and gel drying methods taught in theory, the execution of the isolated proteins by SDS-PAGE gel electrophoresis and Coomassie staining and presented as a report evaluating the results
11) Methods of concentrating proteins, the general principles of chromatography, to isolate the cell components of the protein, cytoplasmic and mitochondrial protein isolated from mammalian cells, quantification by Bradford
12) Analysis of proteins as macromolecules, molecular biological techniques, microarray analysis, protein microarray, EMSA, Mikrosekuanslama, DNA footprinting methods learned in theory, evaluation of results and reports are provided as Western blotting
13) Analysis of proteins as macromolecules, molecular biological techniques, ELISA method, in theory, working principle and types of teaching, learning to estimate protein concentration by ELISA in serum samples, giving results as a report, by the course of molecular biology techniques to investigate their use in the study of molecular biology techniques are committed student research presentations
14) Presentation
15) Presentation
16) Presentation

Recommended or Required Reading

Planned Learning Activities and Teaching Methods

1) Lecture
2) Question-Answer
3) Discussion
4) Drill and Practice
5) Lab / Workshop

Assessment Methods and Criteria

Contribution of Semester Studies to Course Grade	30%
Number Percentage Semester Studies Midterm Examination 1 40% Practices 1 30% Project 1 30%
Contribution of Final Examination to Course Grade	70%
Total	100%

Language of Instruction

Turkish

Work Placement(s)

Not Required