İmmunohistochemistry and Confocal Imaging Techniques In Stem Cells
Stem Cell and Tissue Regeneration
Institute of Health Sciences
Third Cycle (Doctorate Degree)
| Course Unit Title | Course Unit Code | Type of Course Unit | Level of Course Unit | Year of Study | Semester | ECTS Credits |
|---|---|---|---|---|---|---|
| İmmunohistochemistry and Confocal Imaging Techniques In Stem Cells | KHT649 | Elective | Doctorate degree | 2 | Fall | 5 |
Name of Lecturer(s)
Prof. Dr. Yusufhan YAZIR
Learning Outcomes of the Course Unit
1) Can perform staining of the stem cells by immunohistochemical methods.
2) Knows the basic principles of imaging with confocal microscope.
Program Competencies-Learning Outcomes Relation
| Program Competencies | |||||||
| 1 | 2 | 3 | 4 | 5 | 6 | ||
| Learning Outcomes | |||||||
| 1 | No relation | No relation | No relation | No relation | No relation | No relation | |
| 2 | No relation | No relation | No relation | No relation | No relation | No relation | |
Mode of Delivery
Face to Face
Prerequisites and Co-Requisites
None
Recommended Optional Programme Components
...
Course Contents
Stem cells, classification and characterization. Antigen antibody relationship. Immunohistochemical staining methods. Microscope and cell imaging. Confocal microscope and imaging techniques.
Weekly Schedule
1) Description of stem cell2) Stem cell staining tecniques
3) Immunohistochemical staining tecniques in stem cells
4) Direct method
5) Three-Step Indirect Method
6) Avidine-Biotin Method
7) Immunohistochemical staining steps
8) Midterm exam
9) Microscope types
10) Confocal Microscope and its principle of use
11) Types of lasers in a confocal microscope
12) Properties of objectives used in confocal microscopy
13) Fluorescent dyes Used for Confocal Microscope Imaging
14) Imaging of stem cells in confocal microscopy
15) Difficulties encountered in staining and imaging and their solutions
16) Final exam
Recommended or Required Reading
Planned Learning Activities and Teaching Methods
1) Lecture
2) Question-Answer
3) Discussion
4) Lab / Workshop
Assessment Methods and Criteria
Contribution of Semester Studies to Course Grade |
20% |
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Contribution of Final Examination to Course Grade |
80% |
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Total | 100% | |||||||||||
Language of Instruction
Turkish
Work Placement(s)
Not Required