Gene Therapy In Stem Cells
Stem Cell and Tissue Regeneration
Institute of Health Sciences
Third Cycle (Doctorate Degree)
| Course Unit Title | Course Unit Code | Type of Course Unit | Level of Course Unit | Year of Study | Semester | ECTS Credits |
|---|---|---|---|---|---|---|
| Gene Therapy In Stem Cells | KHT648 | Elective | Doctorate degree | 2 | Fall | 5 |
Name of Lecturer(s)
Associate Prof. Dr. Gökhan DURUKSU
Learning Outcomes of the Course Unit
1) Recognizing basic concepts of recombinant DNA technology
2) Using the recombinant DNA technology in the directional differentiation of stem cells
3) Using recombinant DNA technology methods in the applications of tissue regeneration and tissue engineering
4) Evaluating the importance of recombinant DNA technology in stem cell biology and applications with a critical view
5) Gaining critical view on different approaches of gene therapies
Program Competencies-Learning Outcomes Relation
| Program Competencies | |||||||
| 1 | 2 | 3 | 4 | 5 | 6 | ||
| Learning Outcomes | |||||||
| 1 | No relation | No relation | No relation | No relation | No relation | No relation | |
| 2 | No relation | No relation | No relation | No relation | No relation | No relation | |
| 3 | No relation | No relation | No relation | No relation | No relation | No relation | |
| 4 | No relation | No relation | No relation | No relation | No relation | No relation | |
| 5 | No relation | No relation | No relation | No relation | No relation | No relation | |
Mode of Delivery
Face to Face
Prerequisites and Co-Requisites
None
Recommended Optional Programme Components
Not Required
Course Contents
The relation among gene, peptide and protein, modification of DNA and its tools, fundamental methods in recombinant DNA technology, regulation of gene expression, viruses and gene transfer, gene modification in stem cells, cell programming and differentiation, epigenetic and recombination are among the scopes of this course.
Weekly Schedule
1) Genes and proteins2) Gene expression and regulation
3) RNA: From catalytic RNA to miRNA
4) Recombination and transposons
5) Chromosomes and nucleosomes
6) Intracellular signal transduction
7) Signaling pathways and differentiation potential of stem cells
8) Mid-term exam
9) Transcription factors and epigenetics
10) Stem cells and epigenetics
11) Cell differentiation and reprogramming
12) Cell differentiation and reprogramming
13) Cell morphology and cytoskeleton
14) Cellular identity
15) Cellular identity
16) Final exam
Recommended or Required Reading
1- Culture of Human Stem Cells.R. Ian Freshney, Glyn N. Stacey, Jonathan M. Auerbach. 2007, Wiley-Blackwell ISBN: 978-0-470-05246-4
2- Stem cells: scientific progress and future research directions. National Institutes of Health. November 2004, University Press of the Pacific. ISBN: 978-1-4102-1896-4
Planned Learning Activities and Teaching Methods
1) Lecture
2) Question-Answer
3) Discussion
4) Drill and Practice
5) Self Study
Assessment Methods and Criteria
Contribution of Semester Studies to Course Grade |
20% |
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Contribution of Final Examination to Course Grade |
80% |
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Total | 100% | |||||||||||||||||
Language of Instruction
Turkish
Work Placement(s)
Not Required